2 - Prokaryotes and Eukaryotes

• What is a eukaryote?

  An organism with cells that have nucleus and memebrane bound organellles, like mitochondria
• What is a prokaryote?

  An organism with cells that do not contain a true nucleus or other membrane bound organelles
• What is a light microscope?

  Uses light to allow us to see the specimens
• What is an electron microscope?

  Uses electrons to allow us to see specimens
• How does a TEM work?

  A beam of electrons passes through the specimen and is dispersed by the structures there. Scattered electrons are then captured on a photographic plate.
• How does a SEM work?

  The specimen is coasted in a thin layer of metal and a beam of electrons is bounced off the surface onto a photographic plate. 3D images can be formed.
• What is found in a eukaryotic cell? e.g. yeast fungal cell

  Food storage granules, ribosomes, vacuole, cell wall made of chitin, cell membrane, cytoplasm, mitochondria, nucleus
• Name the parts of a prokaryotic cell...

  Cell wall made of peptidoglycan, bacterial DNA, plasmid DNA, cytoplasm, cell membrane, sometimes a flagellum
• Abbrev. of microscope comparison table...

  CCRMAS2
• 1st comparison?

  cost - cheap/expensive, schools/universities or labs
• 2nd comparison?

  Colour = if stained then yes, blakc and white
• 3rd comparison?

  Resolution - up to 0.2 micrometres, up to 0.1 nano metres
• 5th comparison?

  Magnification - up to x2000, up to x2, 000, 000
• 6th comparison?

  Alive or dead - living, dead in a vaccum
• 7th comparison?

  Staining - yes if colourless but not neccesary, YES or can be added later
• 8th comparison?

  2d or 3d - 2D, 3D/2D