Resolution and magnification

• What is the magnification calculation?

  magnification = size of image/actualsize of image
• What is the max magnification of a light microscope?

  x1400
• What is the max magnification of a laser scanning confocal microscope?

  x1400
• What is the max resolution of light microscopes?

  250nm
• What is the max resolution of laser scanning confocal microscopes?

  250nm
• Why do microscopes have a max resolution?

  The wave can only pass through half of its wave length so light is 500nm so it is only able to pass through a gap between cells or something larger than 250nm
• How does resolution effect an image?

  It causes details to be lost the further the image is magnified becoming blurry and not useful so it limits the max magnification that could be used
• What is the max magnification of an electron scanning microscope?

  x980000
• What is the max resolution of a scanning electron microscope?

  10-3nm
• What is the max resolution of a transmitting electron microscope?

  0.1nm
• what is the max magnification of a transmitting electron microscope? 

  x980000
• What are the pros of light microscopes?

  -live specimen

  -easy to use

  -cheap

  -see in colour
• What are the pros of laser scanning confocal microscopes?

  -live specimens

  -depth selectivity
• Pros of transmission electron light microscope

  -highest magnification

  -highest resolution
• Pros of scanning electron microscopes

  -3D image

  -high magnification

  -high resolution
• Cons of light microscopes

  -2D image

  -Low magnification

  -Low resolution
• Cons of laser scanning confocal light microscope

  -Low magnification

  -Low resolution

  -Complex staining

  -Training required
• Cons of transmission electron microscope

  -Training required

  -No colour

  -Dead specimen
• Cons of scanning electron trasmission microscoope

  -No colour

  -Training required

  -Deadspecimen