RP 1

• What are the four factors that affect enzyme activity?

  Enzyme concentration, substrate concentration, temperature, and pH
• How is a control set up in a practical measuring enzyme activity?

  By replacing the enzyme solution with distilled water or boiled enzyme solution
• How can the results of a practical measuring enzyme activity be used to find the initial rate of reaction?

  By plotting results on a graph of ‘rate of reaction’ against ‘time’ and drawing a tangent at time = 0
• What is the practical procedure used to measure the effect of temperature on enzyme activity using trypsin and milk?

  • Immerse equal volumes of trypsin and milk in different test tubes in a water-bath for 5 minutes.
  • Mix together and start timing immediately.
  • Record the time taken for the milk to become colorless.
  • Test at least 5 temperatures with at least 3 repeats at each temperature.
• How is the rate of reaction calculated from time?

  Rate of reaction = 1/time
• What is the effect of temperature on enzyme activity?

  As temperature increases, kinetic energy increases, leading to more ES complexes forming until the optimum temperature is reached
• What happens to the enzyme's active site beyond the optimum temperature?

  Bonds in the enzyme's tertiary structure break, changing the shape of the active site
• What is the risk associated with handling enzymes in a laboratory setting?

  Students may have allergic reactions to enzymes, so contact with skin and eyes should be avoided
• What is the level of risk associated with handling enzymes?

  Low risk