RP 6

• What is one aseptic technique used in experiments?

  Wipe down surfaces with antibacterial cleaner, before and after the experiment
• What is another aseptic technique to prevent contamination?

  Use a Bunsen burner in the workspace to draw microbes away from the culture
• What should be done to the wire hoop before transferring bacteria?

  Flame the wire hoop before using it
• Why should the neck of bottles be flamed before use?

  To prevent any bacteria from entering the vessel
• How long should vessels containing bacteria be kept open?

  For the minimum amount of time
• What should be done with windows and doors during the experiment?

  Close windows and doors to limit air currents
• Why is bacteria incubated at 25°C?

  To prevent the growth of pathogens, which occurs at higher temperatures
• How can you compare the effectiveness of different antibiotics on the same bacteria?

  Measure the diameter and calculate the area of the zone of inhibition on the agar
• What does the zone of inhibition indicate?

  It indicates the bacteria killed by the antibiotic; a larger zone means a more effective antibiotic
• What does it mean if an antibiotic has very little or no zone of inhibition?

  The bacteria is likely resistant to the antibiotic
• What is a hazard associated with using a naked flame in the lab?

  Keep away from flammable materials