RP 11

• How can Benedict’s solution be used to measure the concentration of glucose in a solution?

  By using a colorimeter to measure absorbance and creating a calibration curve.
• What is a serial dilution?

  A dilution where successive concentrations increase or decrease in a logarithmic fashion.
• What are the axes in a calibration curve for glucose concentration measurement?

  Absorbance against glucose concentration.
• What would a high glucose concentration in urine suggest?

  It may suggest diabetes due to lack of insulin leading to high blood glucose concentration.
• What are the hazards and precautions of using Benedict’s solution in this practical?

  • Benedict’s solution is an irritant; wear eye protection.
  • Avoid contact with skin.
  • Handle the hot water bath with care.
• How can you increase the accuracy of the estimate of the unknown glucose solution?

  By increasing the number of concentrations at smaller intervals for the calibration curve.