Secretory Status Determination

• The antigens A and B of the ABO systems are not solely present on the surface of the red cells.
• Those who show the presence of the antigens in the body secretions are called secretors.
• 80% of people secrete these agglutinogens in the body fluids as water soluble form
• Non-secretors are individuals who possess only lipoidal form of these agglutinogens.
• In Non-Secretors blood group, antigens are usually present over tissues like RBC’s epithelium
• The ability to secrete soluble antigens is under control of a pair of genes, Se and se.
• Secretors → SeSe & Sese

  Non-secretors → sese
• Se gene is responsible for the expression of the H antigen on glycoprotein structures located in body secretions
• se se individuals do not convert antigen precursors to H antigen and has neither soluble H antigen nor soluble A or B antigens in body fluids
• There are a many different types of body fluids that are secreted, they can be broken down in two categories: Excreted and Secreted
• In preparing the reagent, anti-sera must be in 1/4 dilution
• In preparing the reagent, reagent red cells must be in 5% suspension
• A) A

  
• A) B, H

  
• A) AB

  
• A) O

  
• A) H

  
• A) A, H

  
• A) B

  
• A) A, B, H

  
• In secretory status determination, an absence of agglutination is a positive result
• Dilution of reagents and samples is to prevent zonal reaction
• Excessive levels of ABH substances leads to ABO discrepancies such as:

  • Pseudo mucinous ovarian cyst
  • Pancreatic & Stomach carcinoma
  • Intestinal obstruction