Module 22: Mass Spectrometry

• What is the purpose of the inlet system in a mass spectrometer?

  The sample is introduced and vaporized by heating.
• How are sample molecules or atoms ionized in a mass spectrometer?


  By knocking out one or more electrons.
• What is the process of electron bombardment in ionization?


  Electrons generated in the glowing filament bombard with the sample molecules and produce a beam of ions.
• What occurs during thermal ionization?


  Atoms get ionized when in contact with a heated metal surface.
• What are the applications of mass spectrometry?


  • Determination of the molecular weight of compounds
  • Analysis of proteins, peptides, lipids, and oligonucleotides
  • Determination of the molecular formula
  • Determination of molecular structure from mass spectral data and fragmentation pattern
• What are the main components of a mass spectrometer's instrumentation?

  • Electron beam source
  • Sample injector
  • Sorting/separating
  • Accelerating slits
  • Heating filament
  • Ionization
  • Magnetic field
  • Ion stream
  • Detector
  • Deflection
  • Recorder
• What is one strength of mass spectrometry?


  It allows for quick analysis.
• What is a limitation of mass spectrometry?


  Distinction of compounds having the same m/z ratio is difficult.