Factors affecting e. Activity

• Temperature

  • Incr in temp →incr in rate of rtn (till opt. Temp.)
  • If > opt. temp → Denaturation →decr act.
  • 4 human enzymes → 36°C-38°C
  • Low 🔅 e. inactive ← avail activation energy ↓
• pH

  • Every e → specific pH
  • Slight change: reduc rate
  • Extreme change Dnatur e
  • Pepsin | pH 2
  • Trypsin | pH 8
  • Both r protein digesting e