The reactions of the light independent stage of photosynthesis
Compartmentalisation
The use of intracellular membranes to separate metabolic processes within the cell e.g. nuclear envelope around the nucleus.
Grana
A stack of thylakoid membranes in chloroplasts.
Light dependent stage
The first stage of photosynthesis, which uses light energy to produce ATP and reduced NADP.
Light independent stage
The second stage of photosynthesis, which used CO2, ATP and reduced NADP to produce organic molecules.
Limiting factor
A variable that limits the rate of a particular process.
NAD
A coenzyme molecule found in all living cells which can be reduced by removing hydrogen atoms and accepting electrons from substrates.
NADP
A coenzyme that is reduced (gains H atoms) in the light dependent stage of photosynthesis and is oxidised (loses H atoms) in the light independent stage.
Photolysis
An enzyme-catalysed reaction of photosynthesis that uses light energy to split water
Photosynthesis
The set of reactions in chloroplasts that use light energy, water and CO2 to produce organic molecules.
Stroma
The fluid-filled matrix of chloroplasts and site of light independent stage of photosynthesis.
Thylakoid
The inner membrane of a chloroplast and site of the light dependent stage of photosynthesis
Stomata
Pores/ holes on the leaf where O2 exits and CO2 enters
Calvin Cycle
Final stage of photosynthesis. Takes place in the stroma of chloroplasts. Produced ATP and reduced NADP.
Carbon Fixation
The conversion of gaseous CO2 into organic molecules.
Rubisco
The enzyme that catalyzes the first step of the Calvin cycle (the addition of CO2 to RuBP).
RuBP
five-carbon carbohydrate that combines with CO2 in the first step of the Calvin Cycle
Limiting Factor
A factor that limits the rate of a process. If the limiting factor is increased the process will take place at a faster rate. This increase in rate does not go on indefinitely. Eventually the rate levels off as other factors become limiting. Light intensity and carbon dioxide concentration are limiting factors for photosynthesis.
Compensation Point
There is a particular level of light intensity at which rate of photosynthesis equals rate of respiration.
ATP
The main energy source for all cells; a molecule that is catabolized to release energy to drive metabolic processes
Where does the light dependent stage occur?
In the grana
Where does the light independent stage occur?
In the stroma
How is the inner membrane adapted for its role?
It is embedded with transport proteins and is less permeable than the outer membrane. This enables control over which substances can enter the stroma from the cell cytoplasm.
How are the thylakoid membranes adapted for their role?
They are stacked which produces a large surface area for the light dependent reaction to occur.
How are the photosynthetic pigments adapted for their role?
They are organised in photosystems which maximise the efficiency of light energy absorption.
How is the grana adapted for its role?
It is surrounded by the stroma meaning products from the light dependent reaction are able to pass directly to the enzymes catalysing the light independent reactions.
How are chloroplasts adapted for their role?
They contain their own DNA and ribosomes which enables them to produce some of their photosynthetic proteins rather than importing them from the cell cytoplasm.
Light energy is absorbed by chlorophyll molecules, which are located within pigment protein complexes called photosystems.
The electrons excited by light move through an electron transport chain (ETC) that uses this energy to pump protons across the thylakoid membrane into the lumen of the thylakoids
NADPH is produced when electrons are passed down another ETC, reducing NADP+ to NADPH