microscopes

• What type of microscopes are compared in the study material?

  Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM)
• What are the similarities between SEM and TEM?


  • Both use electromagnets and electrons
  • Both use vacuums to view specimens, so specimens must be dead
  • Both have higher magnification and resolution than optical microscopes
• What are the differences between SEM and TEM?


  • In TEM, electrons are transmitted through the specimen; in SEM, electrons are reflected off the surface
  • TEM shows internal structure, while SEM shows surface details
  • SEM produces 3D images, whereas TEM cannot
  • TEM has a higher resolution and maximum magnification compared to SEM
  • SEM can be used on thicker specimens than TEM
• How do the imaging techniques of SEM and TEM differ in terms of electron interaction with specimens?


  In TEM, electrons are transmitted through the specimen, while in SEM, electrons are reflected off the surface.
• What type of images does SEM produce compared to TEM?


  SEM produces 3D images, while TEM produces 2D images.
• Which microscope has a higher resolution, SEM or TEM?

  TEM has a higher resolution compared to SEM.
• Can SEM be used on thicker specimens compared to TEM?


  Yes, SEM can be used on thicker specimens than TEM.
• There are two types of electron microscopes to know about:

  Scanning electron microscopes provide a 3D image of the outer surface of a sample.

   

  In contrast, transmission electron microscopes provide a 2D image of the sample’s internal structure.
• Compared to an electron microscope, which of these are benefits of an optical microscope?

  • produces colour images
  • it is more portable
  • can analyse living specimens