What is Microscopy?

• What does microscopy refer to?


  The use of microscopes
• What are the main components of a light microscope?


  The base, arm, light source, stage, objective lenses, eyepiece lens, body tube, and focusing knobs
• What is the function of the base in a light microscope?


  It supports the entire microscope
• What is the purpose of the arm in a light microscope?


  It connects the base to the top of the microscope
• What type of light source can be used in a light microscope?


  A lamp or a mirror that reflects room light
• Where is the stage located on a light microscope?


  Just above the light source
• What is the role of the objective lenses in a light microscope?


  They provide different magnifications for viewing the object
• How many objective lenses are typically found in a light microscope?


  Three objective lenses
• What are the common magnifications of objective lenses?


  10 times, 20 times, and 50 times magnifications
• What is the function of the eyepiece lens in a light microscope?


  It has a fixed magnification and is where we look into the microscope
• What are the coarse and fine focusing knobs used for?


  They help to get the image in focus
• What does the term 'object' refer to in microscopy?


  The real object or sample being observed
• What does the term 'image' refer to in microscopy?


  The visual representation seen when looking through the microscope
• How does light travel through a light microscope?


  It reflects off the mirror, passes through the object, and through the lenses into the eye
• What is magnification in microscopy?


  How many times larger the image is than the object
• What is the equation for calculating magnification?


  Magnification = image size / object size
• What is resolution in microscopy?


  The shortest distance between two points on an object that can still be distinguished as separate
• Why is resolution important in microscopy?


  It determines how detailed the image is and how clear the object appears
• If two parts of an object are too close together, what happens to their visibility in microscopy?


  They may appear blurred and indistinguishable
• How does higher resolution affect the appearance of an image?


  The image will have more details and appear less blurry
• What are the key differences between object and image in microscopy?


  • Object: The real sample being observed (e.g., onion cells)
  • Image: The visual representation seen through the microscope
• What is the process of light travel in a light microscope?


  1. Light hits the mirror and reflects upwards
  2. Light passes through the object on the stage
  3. Light goes through the objective lens
  4. Light passes through the eyepiece lens
  5. Light enters the eye
• What is the relationship between magnification and resolution?

  • Magnification: Size of the image relative to the object
  • Resolution: Clarity and detail of the image
• What happens to the visibility of two points on an object if they are too close together?


  • They may appear blurred and indistinguishable
• How does higher resolution affect the clarity of an image?


  • Higher resolution results in more details and less blurriness