B2 - enzymes/amalyse

• method:

  1. fill test tube with 50cm of starch solution
  2. place test tube in water bath of 35 degrees
  3. add buffer to change PH to 5
  4. add 10cm of amalyse solution using pipette and start stopwatch
  5. every 30 seconds, use pipette to add drop of solution to well plate - add iodine solution to test for starch
  6. stop when starch solution no longer turns blue-black
  7. repeat 3 times at same PH to calculate mean
  8. repeat for different PH levels
  9. plot graph with PH on x axis and time taken to break down starch on y axis
• • control variables are volume of starch solution and volume of amalyse solution
  • keep time intervals same