LAB QUIZ 1

• COMPLEX


  -Also known as non-synthetic, natural

  -Contains materials from natural sources, like yeast extract, meat extract, peptones and proteoses.

  -Exact composition may vary in from one batch to another
• CHEMICALLY-DEFINED 


  -Also referred as synthetic, defined

  -Composed of exact amounts of chemically pure, specifically identified organic or inorganic components
• NUTRIENT MEDIA 


  -Contain all the elements that most bacteria need for growth and are nonselective so they are used for the general cultivation and maintenance of bacteria kept in laboratory culture collections.
• PLATE COUNT AGAR (PCA) 


  -Also called Standard Methods Agar (SMA), is a microbiological growth medium commonly used to assess or to monitor "total" or viable bacterial growth of a sample.
• Nutrient agar


  general-purpose medium supporting growth of a wide range of nonfastidious microorganisms
• Trypticase Soy Agar


  Are general-purpose media, providing enough nutrients to allow for a wide variety of microorganisms to grow.
• Selective Media


  • Are used for the growth of only selected microorganisms
  • Contain inhibitory substances for other types of microorganisms aside from desired ones
• Eosin Methylene Blue


  • Also known as "Levine's formulation“
  • Contains dyes that are toxic for Gram-positive bacteria and bile salt which is toxic for Gramnegative bacteria other than coliforms.
• Yeast and Mold


  Is a selective growth medium with low pH useful for cultivating yeasts, molds, or other acid-tolerant or acidophilic organisms
• MacConkey Agar


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  • designed to selectively isolate Gram-negative and enteric bacilli and differentiate them for lactose fermentation
• Mannitol Salt Agar 


  • Is a commonly used selective and differential growth medium.
  • It encourages the growth of a Staphylococcus spp. while inhibiting the growth of others.
• Differential media 


  distinguish a type of microorganisms from another growing on the same media.
• Tripple Sugar Iron Slant 


  Determine the ability of microorganisms to ferment sugars and to produce hydrogen sulfide
• Blood Agar plate

  • Is enriched and differential medium used to isolate fastidious microorganisms and detect hemolytic activity
• enriched media


  contain the nutrients required to support the growth of a wide variety of microorganisms, including some of the more fastidious ones.
• chocolate agar


  enriched with heat-treated blood (40-45°C), which turns brown and gives the medium the color for which it is named
• specific media


  Allows growth of a particular organism
• reducing media


  • A microbiological growth media that chemically combines with dissolved oxygen and removes the oxygen from the media
• Growth medium or Culture medium


  liquid or gel containing nutrient materials
• Specific media

  • Modified Thayer - Martin VPN
  • Lowenstein Jensen Media