"I hate the Moor, and it is thought abroad that 'twixt my sheets he's done my office".
- Reiterates that he "hates the Moor" in A1, S3 twice, showing the extent to which he hates Othello and implying this his hate towards Othello will act as a motivation for him in pursuing his plans and achieving his goals of bringing Othello's downfall.
- His first soliloquy with the audience - he creates an intimate relationship with the audience and is the only character to do so. Although the audience are aware of his nature and him as a vice character, they may still prefer Iago over the other characters just because he is the only one that has that form of relationship with the audience.
- Iago presented as opportunistic and a character who does not have a plan, rather, his plans are formed based on what he is being revealed, therefore he makes his plans as he goes along. In this case, he states that he hates Othello because Othello might have slept with Emilia - sexual jealousy.
- Sexual jealousy is at the forefront of the play and a key theme of the play. All women of the play suffer at their lovers' hands because of sexual jealousy and are either mistreated (Bianca) or have their lives sacrificed (Desdemona and Emilia).
- Sexual jealousy is a catalyst to the tragedy and the reason as to why there is conflict and violence. Ultimately, men's insecurity causes chaos.
- Iago admits that he has no evidence of this claim - "I know not if't be true" - yet, acts on his suspicion anyway. His unsubstantiated claims suggest that sexual jealousy is just an excuse to cause trouble.
- Shakespeare illustrates how jealousy derives from male ego, as the repetition of the first person possessive "my" conveys Iago's sense of ownership over Emilia and their union.