Effect of pH on amylase

• amylase breaks down starch into simple sugars
• 1

  place 1 drop of iodine into each well of a spotting tile
• 2

  • fill 1 test tube with 2cm cubed starch solution
  • the second with 2cm cubed amylase
  • the third with 2cm cubed pH buffer
• 3

  place the test tubes in a water bath at 30 degrees for 10 minutes so the solutions can reach the right temperatures
• 4


  mix the 3 solutions into 1 test tube then place back into water bath and start stopwatch
• 5

  after 30 seconds transfer a drop of solution to a well of iodine and it will turn blue-black showing starch is present
• 6

  take a sample every 30 seconds and continue until iodine remains orange which shows starch is no longer present
• 7


  repeat with different pH buffers