microscopy

• What should you be able to describe by the end of the video?

  The advantages of an electron microscope over a light microscope
• What is the primary function of microscopes?

  To magnify images of specimens
• What did early microscopes look like?

  They looked much simpler than modern microscopes
• What important discoveries have light microscopes allowed us to make?

  Discoveries about the structures inside cells, such as the nucleus
• What are the limitations of light microscopes regarding magnification?

  They have a limited magnification that cannot easily view structures inside the nucleus
• What does limited resolution in light microscopes mean?

  It means the image is blurred and lacks fine detail
• Why was the electron microscope invented?

  To explore structures inside cells in detail
• What are the key advantages of electron microscopes over light microscopes?

  They have much greater magnification and resolution
• How can you calculate the magnification of a microscope?

  By using the equation: magnification = size of the image / size of the real object
• What is the diameter of the real nucleus in the example given?

  0.01 mm
• If the size of the image of the nucleus is 45 mm, what is the magnification?

  4,500 times
• What is the length of the real mitochondrion in the example given?

  0.3 mm
• If the image size of the mitochondrion is 30 mm, what is the magnification?

  10,000 times
• How do you calculate the size of the real object using magnification?

  By using the equation: size of the real object = size of the image / magnification
• What is the size of the image in the example given for the cell?

  87 mm
• If the magnification is 2,000 times, what is the size of the real cell?

  0.435 mm
• What are the main differences between light microscopes and electron microscopes?

  • Light microscopes have limited magnification and resolution.
  • Electron microscopes have much greater magnification and resolution.
  • Light microscopes use light to view specimens, while electron microscopes use electrons.
• What steps should you follow to calculate magnification?

  1. Measure the size of the image.
  2. Measure the size of the real object.
  3. Use the formula: magnification = size of the image / size of the real object.
• How do you find the real size of an object if you know the image size and magnification?

  1. Measure the size of the image.
  2. Use the formula: size of the real object = size of the image / magnification.