Enzyme Activity

• What is the main focus of the investigation described in the study material?

  The effect of a variable on the rate of enzyme activity
• What is one way to investigate how temperature affects enzyme activity?

  By carrying out a series of different temperature experiments
• How can you control variables when investigating temperature's effect on enzyme activity?

  By using a different heat example to control variables
• What is another variable that can be investigated in relation to enzyme activity?

  The effect of pH
• How can you investigate the effect of pH on enzyme activity?

  By adding a variable offer mixture with a different pH level
• What is an example of how to adjust the pH in an experiment?

  By adjusting tubes containing the enzyme substrate mixture
• What can be varied to investigate the effect of substrate concentration?

  The initial concentration of the substrate
• What is the purpose of varying the total concentration of the enzyme?

  To investigate the effect of enzyme concentration
• What does the enzyme catalyze in the practical investigation?

  The breakdown of hydrogen peroxide into water and oxygen
• How can you measure the rate of product appearance in the enzyme activity investigation?

  By collecting the oxygen produced
• What are the methods to investigate enzyme activity based on the study material?

  • Investigate temperature effects through different temperature experiments
  • Investigate pH effects by adding mixtures with varying pH levels
  • Vary initial substrate concentration to observe its effect
  • Vary total enzyme concentration to study its impact
• What is the process of measuring enzyme activity through product appearance?

  1. Enzyme catalyzes breakdown of hydrogen peroxide
  2. Collect oxygen produced
  3. Measure the rate of oxygen appearance