producing monoclonal antibodies

• 1

  mouse is injected with an antigen causing it to produce lymphocytes which produce antibodies against the antigen
• 2


  lymphocytes are extracted and combined with a tumour cell creating a hybridoma cell
• 3


  the hybridoma cell that produces the antibodies needed in collected and it divides forming clones of the antibody
• 4


  a large amount of them is collected and purified
• monoclonal antibodies are specific to a single binding site on one protein antigen