AUTOMATION

• what is the use of automation

  it is used to increased productivity, higher throughput, higher production rates, as well as efficient use for the reagents and materials. it also reduced errors and turnaround time improved
• electric loop incinerator


  sterilization is obtained by the means of infrared rays
• in electric loop incinerator sterilization is complete when it reaches how many seconds


  5-8 seconds at 850c
• centrifuge


  a motor based and allows the rotation of a liquid sample resulting in the separation of the components of the mixture
• hot plate


  a stand alone appliance used in microbiology laboratories as a TABLE TOP HEATING SYSTEM. it is also commonly used in PREPARING MEDIA
• autoclave


  pressurized chamber used for the process of sterilization and disinfection by combing three factors; time, pressure and steam
• in autoclave what are the 3 factors that are combined

  time, pressure, and steam
• what is the sterilization agent of autoclave

  steam
• what is the principle of autoclave

  steam under pressure
• to be effective, autoclave must reach and maintain a temperature of

  121c for 15mins under atleast 15 psi of pressure
• what is PSI

  pound per square inch
• what is the actual time for autoclaving

  15 mins
• time for autoclaving endospore cleaning

  30mins
• what is the bacteria used for calibrating autoclave

  geobacillus stearothermophilus
• colony counter


  used in food and water technology
• colony counter


  used to estimate the density of liquid culture by counting the number of CFU on agar or culture plates
• colony counter


  counts the number of colonies
• hot air oven


  an electrical device that is used for sterilization of medical equipment using DRY HEAT
• what materials can Hot air oven sterilized

  glassware, metal equipment, powder
• incubator


  a device that is used in the laboratory for the growth and maintenance of microorganisms and culture
• incubator


  it provides an optimal temperature, pressure, moisture, among others things required for the growth of organisms
• biological safety cabinet


  an enclosed, ventilated workspace for safely working with materials contaminated with pathogens that require a defined biosafety levels
• what do you call the filter where sterile air go through on biological cabinet

  high efficiency particulate air (HEPA)
• a shortwave ultraviolet that sterilizes the workstation

  shortwave ultraviolet germicidal lamp
• used to store and preserve medical equipment, food items, blood samples, medicine and injection for a more extended time

  deep freezer
• continuous monitoring system


  a sophisticated instrumentation that are multidisciplinary strategic technologies that often interfaced with computers
• fluorogenic methodology for organism identification and turbidimetric method for susceptibility testing

  Vitek 2compact
• vitek 2 compact can provide a result in as much as how many hours

  5-8 hours
• Fluorogenic methodology - organism identification

  turbidimetric method - susceptibility testing
• BacT/ALERT Microbial Detection System


  utilizes a Colorimetric sensor and Reflected light to monitor the presence and production of carbon dioxide (CO2) that is dissolve in the media
• what chemical element is produced when microorganisms are present in the test sample

  carbon dioxide
• When growth of the microorganisms produces CO2, the color of the gas-permeable sensor installed in the bottom of each culture bottle changes to


  Yellow
• the colorimetric detection system is designed to (2)

  • to detect microorganisms early
  • to provide result that is predictable and stable
• BacT / ALERT - is used for blood culture
• walk away specimen processor (WASP)


  an automated microbiology specimen processing instrument that offers: gram slide prep, broth inoculation, transferring inoculum volume, automated streaking, and automatic antibiotic disk dispensing
• BD Phoenix M50


  automated microbiology system is intended for the in vitro rapid identification and quantitative determination of bacterial antimicrobial susceptibility by minimal inhibitory concentration (MIC
• BD phoenix M50 is also capable of detecting the presence resistance markers linked to hospital acquired infections such as;

  • methicillin resistant staphylococcus aureus
  • vancomycin resistant enterococci
  • extended spectrum beta-lactamase
• a type of balance that is commonly used for the measurement of mass in the sub milligram range

  analytical balance
• made with a measuring pan enclosed in a transparent covering that prevents smalls particles or air currents from getting collected on the pan.

  analytical balance
• It is a gas-fueled single open flame.

  bunsen burner