Microscopy

• What are the two types of microscopes?


  Light microscopes and electron microscopes
• Why are light microscopes considered conventional microscopes?


  Because they are commonly used in classrooms
• What are the advantages of light microscopes?


  They are small, easy to use, and relatively cheap
• What is the resolution limit of light microscopes?


  0.2 micrometers
• What does a resolution of 0.2 micrometers imply about details smaller than this value?


  They will appear blurry
• What types of structures can light microscopes observe?


  Individual cells like onion cells
• What are the characteristics of electron microscopes?


  They are large, expensive, and hard to use
• What is the benefit of using electrons in electron microscopes?


  Electrons have a wavelength of just 0.1 nanometers
• What is the maximum resolution of electron microscopes?


  0.1 nanometers
• How does the resolution of electron microscopes compare to that of light microscopes?


  Electron microscopes have 2000 times better resolution
• What does a smaller resolution value indicate?


  It means you can see tinier details
• What can electron microscopes be used to study?


  Sub-cellular structures like mitochondria
• What type of image can be obtained from an electron microscope?


  Images with much higher magnifications without going blurry
• What are the pros and cons of light and electron microscopes?

  **Light Microscopes:**

  • Pros: Small, easy to use, cheap
  • Cons: Limited resolution (0.2 micrometers)

  
  

  Electron Microscopes:

  • Pros: High resolution (0.1 nanometers), can study sub-cellular structures
  • Cons: Large, expensive, hard to use
• What is the main takeaway regarding light and electron microscopes?


  To compare their pros and cons