Microbiology

    Cards (36)

    • What type of bacterial culture is being used in the practical?
      A safe strain of E. coli
    • Why is it important to handle the bacterial culture as though it were dangerous?
      To maintain safety and prevent contamination
    • What should you do before starting to handle the microbe?
      Wash your hands thoroughly
    • Where should the agar plates be opened during the practical?
      Near the Bunsen burner
    • What is the first step in preparing the agar plate?
      Mark the agar plate into three sections
    • Where should the agar plate be labeled?
      At the bottom, not the lid
    • Why is it important to write the date on the agar plate?
      To know how old the culture is
    • What should you do if you accidentally touch a non-sterile surface?
      Wash your hands again
    • What does the blue flame of the Bunsen burner create?
      A sterile environment
    • How is the sterile pipette sterilized?
      Wrapped in red paper and heated in an oven
    • What is the correct way to handle the pipette?
      Hold it by the blunt end
    • What should you do after taking a sample from the bacteria bottle?
      Flame the lid before closing it
    • How should the bacterial culture be applied to the agar plate?
      With the lid open for the minimum amount of time
    • What is the purpose of spreading the bacteria over the agar plate?
      To ensure even growth across the plate
    • What happens if you break the surface of the agar while spreading?
      It will be very obvious and affect growth
    • What is the purpose of pouring disinfectant into the beaker?
      To kill any remaining bacteria on the spreader
    • What is the expected outcome when bacteria grow on the agar plate?
      A lawn of bacteria will form, making the agar cloudy
    • What is the purpose of using antibiotic and antiseptic disks in the experiment?
      To test their effectiveness in stopping bacterial growth
    • What is the name of the antibiotic used in the experiment?
      Streptomycin
    • Why is it important to label the disks on the agar plate?
      To identify which disk contains which substance
    • What should be done after placing the disks on the agar plate?
      Incubate the plate for a specific time
    • What indicates that the disks have killed bacteria on the agar plate?
      Clear rings around the disks
    • How can the effectiveness of the treatments be compared?
      By measuring the diameter of the clear areas
    • What factors should be considered to ensure a fair test?
      The viscosity of the liquids and diffusion through the agar
    • How can the results of this experiment be applied to real-world situations?
      To determine effective treatments for bacterial infections
    • What should be done with the agar plate after the experiment?
      Disinfect the table and return the plate to the teacher
    • What is the significance of the tea tree oil in the experiment?
      It is tested for its antibacterial properties
    • Why should agar plates be stored upside down?
      To prevent condensation from affecting the bacteria
    • What happens to the agar plate after a few days of incubation?
      The agar becomes cloudy due to bacterial growth
    • What do the clear areas around the disks indicate?
      That the substances have inhibited bacterial growth
    • How can the results be shared among the class?
      By repeating the experiment and comparing results
    • What is a potential issue with the experiment regarding the antibiotic?
      It was dry while the others were wet
    • What should be considered when interpreting the results of the experiment?
      How the substances diffused through the agar
    • What is the best treatment for a bacterial infection based on the experiment?
      Tea tree oil showed the best effectiveness
    • What are the steps to prepare and inoculate an agar plate with bacteria?
      1. Wash hands thoroughly.
      2. Prepare agar plates and label them.
      3. Open the agar plate near a Bunsen burner.
      4. Inoculate the plate with bacteria using a sterile pipette.
      5. Spread the bacteria evenly using a sterile spreader.
      6. Place antibiotic or antiseptic disks on the plate.
      7. Incubate the plate and observe results.
    • What are the strengths and weaknesses of using different antibacterial substances in the experiment?
      Strengths:
      • Allows comparison of effectiveness.
      • Provides insight into alternative treatments.

      Weaknesses:
      • Different forms (dry vs. wet) may affect results.
      • Results may not reflect real-world effectiveness.
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