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Biology required practicals
Microbiology
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Ellie Norton
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Cards (36)
What type of bacterial culture is being used in the practical?
A safe
strain
of
E. coli
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Why is it important to handle the bacterial culture as though it were dangerous?
To maintain safety and prevent
contamination
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What should you do before starting to handle the microbe?
Wash your hands
thoroughly
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Where should the agar plates be opened during the practical?
Near the
Bunsen burner
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What is the first step in preparing the agar plate?
Mark the agar plate into three
sections
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Where should the agar plate be labeled?
At the
bottom
, not the
lid
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Why is it important to write the date on the agar plate?
To know how old the
culture
is
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What should you do if you accidentally touch a non-sterile surface?
Wash your
hands
again
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What does the blue flame of the Bunsen burner create?
A
sterile
environment
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How is the sterile pipette sterilized?
Wrapped in red paper and heated in an
oven
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What is the correct way to handle the pipette?
Hold it by the
blunt
end
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What should you do after taking a sample from the bacteria bottle?
Flame
the lid before closing it
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How should the bacterial culture be applied to the agar plate?
With the lid open for the
minimum
amount of time
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What is the purpose of spreading the bacteria over the agar plate?
To ensure
even growth
across the plate
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What happens if you break the surface of the agar while spreading?
It will be very obvious and affect
growth
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What is the purpose of pouring disinfectant into the beaker?
To kill any remaining
bacteria
on the spreader
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What is the expected outcome when bacteria grow on the agar plate?
A
lawn
of bacteria will form, making the agar cloudy
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What is the purpose of using antibiotic and antiseptic disks in the experiment?
To test their effectiveness in stopping
bacterial
growth
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What is the name of the antibiotic used in the experiment?
Streptomycin
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Why is it important to label the disks on the agar plate?
To identify which disk contains which
substance
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What should be done after placing the disks on the agar plate?
Incubate
the plate for a
specific
time
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What indicates that the disks have killed bacteria on the agar plate?
Clear rings
around the disks
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How can the effectiveness of the treatments be compared?
By measuring the
diameter
of the clear areas
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What factors should be considered to ensure a fair test?
The
viscosity
of the liquids and
diffusion
through the
agar
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How can the results of this experiment be applied to real-world situations?
To determine effective
treatments
for
bacterial
infections
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What should be done with the agar plate after the experiment?
Disinfect
the table and return the plate to the
teacher
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What is the significance of the tea tree oil in the experiment?
It is tested for its
antibacterial
properties
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Why should agar plates be stored upside down?
To prevent condensation from affecting the
bacteria
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What happens to the agar plate after a few days of incubation?
The agar becomes cloudy due to
bacterial growth
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What do the clear areas around the disks indicate?
That the substances have
inhibited
bacterial
growth
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How can the results be shared among the class?
By repeating the
experiment
and
comparing
results
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What is a potential issue with the experiment regarding the antibiotic?
It was dry while the others were
wet
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What should be considered when interpreting the results of the experiment?
How the
substances
diffused
through the
agar
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What is the best treatment for a bacterial infection based on the experiment?
Tea tree oil
showed the best effectiveness
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What are the steps to prepare and inoculate an agar plate with bacteria?
Wash hands thoroughly.
Prepare agar plates and label them.
Open the agar plate near a
Bunsen burner
.
Inoculate the plate with bacteria using a
sterile
pipette.
Spread the bacteria evenly using a sterile spreader.
Place
antibiotic
or antiseptic disks on the plate.
Incubate
the plate and observe results.
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What are the strengths and weaknesses of using different antibacterial substances in the experiment?
Strengths:
Allows comparison of
effectiveness
.
Provides insight into
alternative
treatments.
Weaknesses:
Different forms (dry vs. wet) may affect
results
.
Results may not reflect
real-world
effectiveness.
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