Rate of reaction RQ

Cards (8)

  • Equipment
    Starch solution
    Amylase solution
    Iodine solution
    Buffered solutions with a range of pH
    Spotting tiles
  • Buffered pH and starch solutions should reach 30 degrees(C) in the water bath
  • one drop of iodine solution into each depression of the spotting tile
  • add 2 cm^3 starch to the amylase and buffer solution then start the timer
  • The iodine should turn black / blue
  • remove a drop every 10 seconds and place it on the spotting tile with the iodine
  • rinse the glass rod before removing the next drop
  • repeat the process with solutions of other pHs