Rate of reaction RQ

• Equipment

  Starch solution

  Amylase solution

  Iodine solution

  Buffered solutions with a range of pH

  Spotting tiles
• Buffered pH and starch solutions should reach 30 degrees(C) in the water bath
• one drop of iodine solution into each depression of the spotting tile
• add 2 cm^3 starch to the amylase and buffer solution then start the timer
• The iodine should turn black / blue
• remove a drop every 10 seconds and place it on the spotting tile with the iodine
• rinse the glass rod before removing the next drop
• repeat the process with solutions of other pHs