Culturing microorganisms

• What is the process by which new cells repair damage?

  Binary fission
• What happens to the cytoplasm during binary fission?

  The cytoplasm divides and forms two cells
• What is the purpose of preparing vaccinations using specific techniques?

  To ensure the safety and effectiveness of the vaccine
• How do cells replicate their DNA during binary fission?

  The DNA replicates and the copies move apart to the ends of the cell
• How long can the binary fission process take?

  As little as 20 minutes
• What must be done to inoculating loops before use?

  They must be sterilized by passing through a hot flame
• Why is it important to store petri dishes at 25°C?

  To prevent the growth of harmful pathogens
• Why is it important to secure the lid of the petri dish with adhesive tape after spreading bacteria?

  To prevent airborne microorganisms from entering
• How should petri dishes be stored to prevent contamination?

  Stored upside down
• What are the key steps in preparing and storing bacterial cultures?

  • Sterilize inoculating loops before use
  • Spread bacteria on agar plates
  • Secure petri dish lids with adhesive tape
  • Store petri dishes upside down
  • Maintain storage temperature at 25°C