Monoclonal antibodies

• What are monoclonal antibodies?

  Antibodies produced from a single clone of cells.
• How are monoclonal antibodies produced?

  By simulating a mouse to produce antibodies against a specific antigen.
• What is done after the mouse's lymphocytes produce antibodies?

  The lymphocytes are combined with a tumor cell.
• What is a hybridoma?

  A cell that can produce antibodies and divide rapidly.
• What is the significance of cloning a single hybridoma cell?

  It produces many identical cells that produce the same antibody.
• What can be done with the large amount of antibody produced from hybridomas?

  It can be collected and purified for use.
• What are the steps involved in producing monoclonal antibodies?

  1. Simulate a mouse to produce antibodies against a specific antigen.
  2. Combine lymphocytes with a tumor cell to form hybridomas.
  3. Clone a single hybridoma cell to produce identical cells.
  4. Collect and purify the large amount of antibody produced.