vibrio

• What family does Vibrionaceae belong to?

  Non-Enteric Gastrointestinal Pathogen
• Why is Vibrionaceae not part of Enterobacteriaceae?

  Because Vibrionaceae is oxidase (+)
• What is the natural habitat of Vibrionaceae?

  Water
• Where is Vibrionaceae commonly found?

  In brackish or salt water
• What shape is Vibrionaceae?

  Comma/curved bacillus
• What type of anaerobe is Vibrionaceae?

  Short, facultative anaerobe
• What does Vibrionaceae ferment?

  Glucose
• What does NLF stand for in relation to Vibrionaceae?

  Non-lactose fermenter
• What does Vibrionaceae reduce nitrates to?

  Nitrites
• Which Vibrionaceae species does not reduce nitrates?

  1. metschnikovii
• What is the indole reaction for Vibrionaceae?

  Indole (+)
• What does the string test indicate for Vibrionaceae?

  String test (+)
• How is Vibrionaceae motile?

  Monotrichous
• What is the halophilic nature of Vibrionaceae?

  Halophilic except V. cholerae and V. mimicus
• What ability do Vibrio parahaemolyticus and Vibrio alginolyticus have?

  Ability to swarm in solid media
• What does a positive string test indicate?

  Formation of stringlike structures
• What is the reagent used in the string test?

  0.5% sodium deoxycholate
• What does a positive string test result indicate about Vibrio species?

  Differentiates Vibrio from Aeromonas species
• What risk factors are associated with Vibrio infections?

  Foreign travel and contact with water
• What is the main symptom of cholera?

  Rice watery stool
• What is the transport medium for Vibrio cholerae?

  Cary blair
• What is the enrichment medium for Vibrio cholerae?

  Alkaline peptone water
• What does TCBS agar indicate for Vibrio species?

  Yellow for sucrose fermenter, green for non-sucrose fermenter
• What is the pH of TCBS agar?

  1. 8.6
• Which Vibrio species are sucrose fermenters?

  1. cholerae and V. alginolyticus
• What are the three major serotypes of Vibrio cholerae O1?

  Inaba, Hikojima, Ogawa
• What is the characteristic of Vibrio cholerae 0139?

  Non-toxigenic and no choleragen produced
• What does Vibrio cholerae Non 01 cause?

  Mild diarrhea
• What is the distinguishing feature of Group 1 Vibrio species?

  Growth in culture media with 0% NaCl
• What is the distinguishing feature of Group 2 Vibrio species?

  Strong reaction in media with 1% to 6% NaCl
• What is the distinguishing feature of Group 3 Vibrio species?

  Strong reaction in myo-inositol fermentation
• What is the distinguishing feature of Group 4 Vibrio species?

  Negative decarboxylase and dihydrolase test
• What is the distinguishing feature of Group 5 Vibrio species?

  Positive with arginine dihydrolase test
• What is the distinguishing feature of Group 6 Vibrio species?

  Strongly positive with LDG
• What is the laboratory diagnosis method for Vibrio species?

  Culture on TCBS agar
• What does a positive Cholera Red Test indicate?

  Red color after adding H2SO4
• What is Pfeiffer’s phenomenon?

  Bacteriolysis of V. cholerae in guinea pig
• What is the purpose of the Vibriostatic test?

  Differentiate Vibrio cholerae 01 and Non 01
• What is the Kanagawa phenomenon associated with?

  Hemolysin production and virulence
• What is the treatment for cholera?

  Doxycycline, azithromycin, or ciprofloxacin