Factors affecting

• what are the factors affecting enzymes

  temperature

  concentration

  pH levels
• what effect does a low temp have on enzymes

  lower kinetic

  lower frequency of successful collisions

  less enzyme substrate complexes formed
• what effect does a high temperature have on enzymes

  overcomes bonds holding the tertiary structure of the enzymes together

  denatures

  cannot form enzyme substrate complex

  lower frequency of successful collisions
• why does pH affect enzyme activity

  enzymes are proteins

  consists of H bonds in tertiary structure

  pH is a measure of the amount of H ions in a solution

  H ions will interact with the H bonds changing the shape of the enzyme and therefore the active site
• are the changes to enzyme action reversible

  temp- no

  pH - sometimes

  concentration - yes
• what are the changes you can make to concentration

  change concentration of enzymes

  change concentration of substrates
• what effect does changing the concentration of enzymes have

  more enzymes results in faster rate

  more successful collisions etc

  less enzymes result in a slower rate

  less successful collisions etc
• what effect does increasing the concentration of substrates have on rate

  more substrates results in a faster rate as more successful collisions occur etc

  less substrates result in a slower rate as less successful collisions occur
• what is the term used when substrate concentration is no longer the limiting factor

  the solution is ' saturated '