VIBRIO

• What shape are Vibrio bacteria?

  1. shaped gram-negative rods
• What diseases are caused by Vibrio species?

  Intestinal diseases, sepsis, and wound infections
• What type of flagella do Vibrio bacteria have?

  Polar flagella
• What compound are Vibrio species sensitive to?

  0129 vibrio static compound
• Why can't Vibrio grow in zero percent salt?

  They are found in ocean environments
• What are the common Vibrio pathogens encountered?

  • V. cholerae
  • V. parahaemolyticus
  • V. alginolyticus
  • V. vulnificus
• What is the minimum salt concentration needed for Vibrio growth?

  Six percent salt
• What specimens are commonly submitted for V. parahaemolyticus and V. cholerae culture?

  • Feces
  • Rectal swab
• What specimens are commonly submitted for V. vulnificus culture?

  • Wound culture
  • Blood
• What culture media are used for Vibrio species?

  • Blood agar
  • Chocolate agar
  • McConkey agar
  • TCBS agar
• What does a pink color indicate on McConkey agar?

  Lactose fermenter (V. vulnificus)
• What does no color change on McConkey agar indicate?

  Non-lactose fermenter
• What is the oxidase test result for all Vibrio species?

  All are oxidase positive
• What indicates a positive oxidase test?

  Purple color in 5-10 seconds
• What does a negative oxidase test indicate?

  No color change
• What does the TSI test determine for Vibrio species?

  If lactose fermenting or not
• What does the VP test differentiate?

  Color change indicating positive or negative
• What is the incubation temperature and duration for nutrient broth with salt?

  35 degrees Celsius for 18-24 hours
• What is the catalase test result for Vibrio species?

  Catalase positive
• What is the decarboxylase test result for V. cholerae?

  Positive
• What shape are Aeromonas bacteria?

  Straight gram-negative rods
• What are the oxidase and indole test results for Aeromonas?

  Oxidase and indole positive
• What is the hemolytic activity of Aeromonas on blood agar?

  Beta hemolytic
• What is the lactose fermentation result for Aeromonas?

  Slightly late lactose fermenter (orange)
• What are the common pathogens of Aeromonas?

  • Aeromonas hydrophila
  • Aeromonas caviae
• How is Aeromonas hydrophila acquired?

  From water or animal meat
• What is the VP test result for Aeromonas hydrophila?

  Positive
• What is the cellobiose test result for Aeromonas hydrophila?

  Negative
• What is the cellobiose test result for Aeromonas caviae?

  Positive
• What specimens are commonly submitted for Aeromonas caviae culture?

  • Feces
  • Rectal swab
• What specimens are commonly submitted for Aeromonas hydrophila culture?

  • Wound culture
  • Blood
• What shape are Plesiomonas bacteria?

  Curved or S-shaped gram-negative rods
• Where can Plesiomonas be found?

  In blood, spinal fluid, and feces
• What is the oxidase test result for Plesiomonas?

  Positive
• What specimens are commonly submitted for Plesiomonas culture?

  • Blood
  • Spinal fluid
  • Feces
• What test is used for Plesiomonas identification?

  Inositol test
• What shape are Campylobacter bacteria?

  Curved or S-shaped gram-negative rods
• What is the appearance of Campylobacter in gram stain?

  Seagull wing shape
• What are the oxidase and catalase test results for Campylobacter?

  Oxidase positive, catalase positive
• What is the motility and oxygen requirement for Campylobacter?

  Motile and microaerophilic