cell fractionation

• step one of cell fractionation


  homogenisation
• homogenisation consists of


  breaking the cells to break the cell membrane and release organelles into the solution using a blender
• the solution needs to be..

  ice cold, isotonic, buffered
• why does the solution need to be ice cold

  to reduce the activity of enzymes that break down organelles
• why does the solution need to be isotonic

  prevents damage to the organelles through osmosis
• why does the solution need to be buffered


  to maintain the pH