Core Practical 2

• What is DCPIP?

  A redox indicator dye
• What color does DCPIP turn when reduced?

  Colorless
• What role does Vitamin C play in relation to DCPIP?

  It acts as an antioxidant
• How can DCPIP be used in experiments?

  To measure Vitamin C content in fruit juice
• What equipment is needed for the DCPIP experiment?

  • DCPIP solution
  • Vitamin C solution
  • Fruit juices
  • Test tubes
  • Pipette
• What is the first step in the DCPIP experiment method?

  Transfer 1cm of DCPIP solution into a test tube
• What should you do after adding Vitamin C solution to DCPIP?

  Shake after each drop
• What should be recorded during the DCPIP experiment?

  The volume of Vitamin C used
• What should be done after the first experiment with Vitamin C?

  Repeat with fruit juices
• What are the hazards and safety precautions in the DCPIP experiment?

  Hazards:

  • DCPIP: Irritant to skin and eyes
  • Broken glass: Cuts from sharp objects
  • Bags/stools: Tripping hazards

  
  

  Safety Precautions:

  • Wear eye protection
  • Handle glassware carefully
  • Keep bags/stools away from workspace
• What should you do in case of DCPIP contact with skin or eyes?

  Wash immediately with cold water
• What is the risk associated with broken glass?

  Cuts from sharp objects
• What should you do if you get cut by broken glass?

  Elevate cuts and apply pressure
• What should be done to avoid tripping hazards?

  Keep bags/stools under desks
• What type of graph should be plotted in the experiment?

  • Absorbance against ethanol concentration
  • Absorbance against temperature
• What is the purpose of plotting a graph in the experiment?

  To analyze the relationship between variables