Vibrio

• Vibrio vulnificus


  Lactose positive
• Common isolates


  V. cholerae O1 and

  non-O1, V. parahaemolyticus, V. vulnificus

  and V. alginolyticus
• Vibrio Mode of acquisition

  Consumption of raw

  or undercooked seafood
• Vibrio Microscopy


  Gram-negative, short, curved,

  asporogenous rods.
• Comma-shaped or curved bacillus


  Vibrio
• Vibrio is Halophilic organisms except:

  Vibrio cholerae

  Vibrio mimicus
• Vibrio cholerae causative agent


  Cholera
• rapid darting/ shooting start motility

  Vibrio cholerae
• Common cause of cholera


  Vibrio cholera 01
• acute diarrheal infection that is mainly spread through contaminated water sources


  Cholera
• Hallmark of cholera

  Rice-watery stool
• protein toxin produced mainly by V. cholerae O1 strai


  Choleragen
• Second most common Vibrio species that is associated with gastroenteriti


  Vibrio parahaemolyticus
• etiologic agent of the outbreak of “summer diarrhea


  Vibrio parahaemolyticus
• Selective medium of Vibrio parahaemolyticus


  wagatsuma agar (high salt mannitol medium)
• Known as the “lactose-positive” Vibrio species


  Vibrio vulnificus
• infection of Vibrio vulnificus


  Septicemia

  Wound infection
• Vibrio species that is commonly isolated and is considered as the least pathogenic to humans


  Vibrio alginolyticus
• Vibrio alginolyticus Strict halophile that requires a medium with:


  1% to 10%NaCl
• Vibrio alginolyticus infection 


  Eye,

  ear

  wound infections.
• LABORATORY DIAGNOSIS of Vibrio
• Laboratory diagnosis of Vibrio


  1. Gram stain
  2. Culture
  3. String test
  4. Vibriostatic test
  5. Biochemical test