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BACTE LAB
vibrios
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Cards (48)
Which vibrios are not halophilic?
Vibrio cholerae
and
Vibrio mimicus
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How are vibrios primarily transmitted?
Through contaminated water or seafood
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Where are vibrios commonly isolated?
In the
gastrointestinal tract
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What is the shape of vibrios?
Comma shaped
or
curved rods
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Are vibrios facultative anaerobes?
True
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Which vibrio is not oxidase positive?
Vibrio
metchnikoii
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What enrichment media is used to isolate vibrios?
Alkaline peptone water
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What agar is used for subculturing vibrios?
TCBS
agar
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What is the initial pH for vibrio isolation?
8.4
pH
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Can vibrios grow in an acidic environment?
False
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What color do sucrose fermenters turn on TCBS agar?
Yellow
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Which vibrios are sucrose positive?
Cholerae
,
alginolyticus
,
fluvialis
,
furnissi
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Which vibrios are sucrose negative?
Parahaemolyticus
,
vulnificus
,
mimicus
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What is the causative agent of cholera?
Vibrio cholerae
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What type of vibrio is halotolerant and non-halophilic?
Vibrio cholerae
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What is the poorly immunogenic antigenic structure?
Flagellar (H)
antigen
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What is the serologically specific antigenic structure?
Somatic (O
) antigen
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Which vibrio cholerae strain causes epidemic and pandemic cholera?
O1
strain
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Which vibrio cholerae strain causes cholera-like disease?
Non-O1
strain
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What are the three serotypes of the O1 strain?
Ogawa
,
Inaba
,
Hikojima
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Where is Ogawa isolated?
India
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Where is Inaba isolated?
Philippines
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Where is Hikojima isolated?
Japan
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What are the two biotypes of the epidemic strain of vibrio cholerae?
Classical
and
El Tor
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Which biotype is responsible for the recent pandemic?
El Tor
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What is the classical biotype's characteristics?
Nonhemolytic
,
vg negative
,
polymyxin B susceptible
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What is the El Tor biotype's characteristics?
Beta hemolytic
,
vg positive
,
polymyxin resistant
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Which vibrio is very similar to cholerae O1 El Tor biotype?
Vibrio cholerae 0139
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What is needed to differentiate O1 from O139?
Antigen detection or polysaccharide capsule detection
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What is an O1 Ag negative and polysaccharide capsule positive vibrio?
Vibrio cholerae O139
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What is an O1 Ag positive and polysaccharide capsule negative vibrio?
Vibrio cholerae O1
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Is Vibrio cholerae only pathogenic to humans?
True
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What is the normal gastric acidity for infection to occur?
More than
1
0
10
10^{10}
1
0
10
vibrio cholerae in water
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What is the organism count in food for infection to occur?
Less than
1
0
2
−
1
0
4
10^{2} - 10^{4}
1
0
2
−
1
0
4
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From where are specimens usually taken for Vibrio cholerae diagnosis?
Stool
and
rectal swabs
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What is used for Vibrio sp transport?
Cary Blair
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What culture media is used for vibrio isolation?
Peptone agar
,
BAP at 9pH
,
TCBS
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Which vibrio species is ADH test positive?
Fluvialis
,
Furnissi
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Which vibrio species is cellobiose test positive?
Vulnificus
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Which vibrio species is VP test positive?
Alginolyticus
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