amylase

• What is the due date for the independent study task?

  14/11/2024
• What is the outcome of identifying variables in an experiment?

  To test a hypothesis
• What is the role of enzymes in biological reactions?

  They speed up reactions without being used up
• What is the specifically shaped part of an enzyme called?

  Active site
• What is the substrate for amylase?

  Starch
• What does the term 'denature' refer to in enzymes?

  When the active site changes shape
• What is continuous sampling in an experiment?

  Regular readings taken throughout the experiment
• What is the effect of pH on amylase activity?

  It affects the rate of enzyme action
• What reagent is used to test for the presence of starch?

  Iodine
• What color change indicates that starch is still present?

  From orange to blue/black
• What does it mean if iodine stays orange during the experiment?

  Starch has been broken down by amylase
• What is the independent variable in the amylase experiment?

  The pH of the amylase solution
• What is the dependent variable in the amylase experiment?

  The time it takes for iodine to stay orange
• What is a control variable in an experiment?

  A variable kept constant throughout the experiment
• How can temperature be controlled in the amylase experiment?

  Using a water bath
• What is the purpose of removing a sample every 30 seconds?

  To test it with iodine for starch presence
• What is the method of measuring time in the experiment?

  Continuous sampling every 30 seconds
• What are the steps to investigate the effect of pH on amylase activity?

  1. Prepare pH buffer solution
  2. Add amylase to the solution
  3. Add starch and start the stopwatch
  4. Take samples every 30 seconds
  5. Test samples with iodine
  6. Record color change
• How do you calculate the rate of reaction in the amylase experiment?

  Rate = 1 / Time (s)
• How should results be expressed in the experiment?

  To three decimal places
• What should be included when plotting the graph of results?

  • Linear scales on both axes
  • Fully labeled axes including units
  • Correct plotting of points
  • A line that best fits the points
• What is the expected color change when starch is digested by amylase?

  From black to orange
• How many values of the independent variable are needed for a line graph?

  5
• Why do enzymes not work when denatured?

  The substrate cannot bind in the active site
• What happens to enzymes at high temperatures?

  They are denatured
• Which enzyme acts on starch to break it into glucose?

  Amylase
• What is the effect of pH on enzyme activity?

  It can increase or decrease activity
• What is the significance of using iodine in the experiment?

  It indicates the presence of starch
• How does continuous sampling improve the experiment?

  It provides regular data points for analysis
• What is the purpose of the control variables in the experiment?

  To ensure a fair test
• What is the expected outcome of the experiment?

  To determine how pH affects amylase activity
• How can the results be interpreted from the graph?

  By analyzing the relationship between pH and enzyme activity
• What should be done after completing the experiment?

  Complete the exam questions 1 and 2