microscopy

• What are the main components of a light microscope?

  Base, arm, light source, stage, lenses
• What is the function of the light source in a microscope?

  To illuminate the object being viewed
• What is the stage of a microscope used for?

  To hold the microscope slide
• What is magnification in microscopy?

  How many times larger the image is than the object
• What is the equation for magnification?

  Magnification = image size / object size
• What is resolution in microscopy?

  The shortest distance between distinguishable points
• How does resolution affect the image quality?

  Higher resolution means more detail and less blur
• What are the key terms related to microscopy?

  • Object: Real sample being observed
  • Image: Visual representation seen through the microscope
  • Magnification: Size of image compared to object
  • Resolution: Shortest distance between distinguishable points
• What is the relationship between magnification and resolution?

  • Magnification increases size of image
  • Resolution determines clarity and detail
  • Higher magnification does not guarantee higher resolution
• How does light travel through a light microscope?

  It reflects off the mirror, through the object, and lenses
• How does resolution affect the clarity of an image?

  Higher resolution means more detail and less blur
• What happens to the image if the resolution is low?

  The image appears blurry with less detail
• Why are light microscopes considered conventional microscopes?

  They are commonly used in classrooms
• What is a key advantage of light microscopes?

  They are small, easy to use, and cheap
• What types of structures can light microscopes not effectively study?

  Sub-cellular structures like mitochondria
• What are the characteristics of electron microscopes?

  They are big, expensive, and hard to use
• What type of structures can electron microscopes study effectively?

  Sub-cellular structures like mitochondria
• What are the pros and cons of light and electron microscopes?

  Light Microscopes:

  • Pros: Small, easy to use, cheap
  • Cons: Limited resolution (0.2 micrometers)

  
  

  Electron Microscopes:

  • Pros: High resolution (0.1 nanometers)
  • Cons: Large, expensive, hard to use