Microscopy

• What is the first step in using a microscope?

  Start with the lowest-powered lens
• Why should you move the stage up with the coarse adjustment knob?

  To bring the specimen into view
• What should you do after adjusting the focus with the coarse adjustment knob?

  Use the fine adjustment knob
• What is the purpose of swapping to a higher-powered lens?

  To see the slide with greater magnification
• What should you use to draw your observations?

  A sharp pencil
• What are the key features to include when drawing observations?

  Unbroken lines, labels, and magnification scale
• If the magnification is x 100, what is the relationship to the real length?

  Magnified length = real length x 100
• What are the main components of a microscope setup for observing specimens?

  • Eyepiece
  • Objective lenses
  • Coarse adjustment knob
  • Fine adjustment knob
  • Stage
  • Stage light
  • Cover slip
  • Specimen stained slide
• What type of cells are mentioned in the study material?

  Onion cells
• What part of the onion cells is specifically mentioned?

  Nucleus
• What structure is noted in the onion cells besides the nucleus?

  Cell wall
• What is the term used for the movement of water across a membrane?

  Osmosis
• What is the effect of osmosis on cells?

  It regulates water balance in cells