Five steps to Measure Rate of Water Uptake

• What is the first step to measure the rate of water uptake?

  Assemble the potometer underwater
• How should the shoot be cut for the water uptake measurement?

  At a slant underwater
• Why is it important to keep the capillary tube submerged when removing the potometer?

  To prevent air from entering the system
• What is done after removing the capillary tube from the beaker of water?

  Form a single air bubble
• What happens to the bubble as the plant transpires?

  The bubble moves along the scale
• What are the five steps to measure the rate of water uptake?

  1. Assemble the potometer underwater.
  2. Cut the shoot at a slant underwater.
  3. Remove the apparatus from the water, keeping the capillary tube submerged.
  4. Remove the capillary tube until a single air bubble forms, then replace it.
  5. Observe the bubble movement as the plant transpires.