Preparing a slide

• What is necessary to view a specimen under a light microscope?

  You need to prepare a microscope slide
• What is a microscope slide made of?

  Clear glass or plastic
• What are the steps to prepare a slide for onion cells?

  1. Add a drop of water to a clean slide.
  2. Cut onion and peel epidermal tissue.
  3. Place tissue in water on the slide.
  4. Add a drop of iodine solution.
  5. Place a cover slip on top.
• What is the purpose of iodine solution when preparing a slide?

  It is a stain to highlight objects
• What should you avoid when placing a cover slip on the slide?

  Getting air bubbles under the cover slip
• What are the steps to use a light microscope to view a slide?

  1. Clip the slide onto the stage.
  2. Select the lowest objective lens.
  3. Move the stage up using the coarse adjustment knob.
  4. Look down the eyepiece and adjust the stage downwards.
  5. Use the fine adjustment knob for clarity.
  6. Swap to a higher-powered lens if needed.
• What is the first step in using a light microscope?

  Clip the slide onto the stage
• What should you do after selecting the lowest objective lens?

  Move the stage up to just below the lens
• How do you achieve a clear image when using a light microscope?

  Use the fine adjustment knob
• What should you do if you need greater magnification?

  Swap to a higher-powered objective lens
• What is the purpose of the coarse adjustment knob?

  To move the stage for initial focusing
• What is the purpose of the fine adjustment knob?

  To achieve a clear image
• How does the process of preparing a slide differ from using a light microscope?

  Preparing a slide involves specimen placement, while using a microscope involves focusing