Enzyme activity: enzyme concentration

    Cards (13)

    • Enzyme Activity: Enzyme Concentration
      • Enzyme concentration affects the rate of reaction
      • The higher the enzyme concentration in a reaction mixture, the greater the number of active sites available and the greater the likelihood of enzyme-substrate complex formation
      • As long as there is sufficient substrate available, the initial rate of reaction increases linearly with enzyme concentration
      • If the amount of substrate is limited, at a certain point any further increase in enzyme concentration will not increase the reaction rate as the amount of substrate becomes a limiting factor
    • How does enzyme concentration affect the rate of reaction?
      Higher enzyme concentration increases reaction rate
    • Why does a higher enzyme concentration increase the likelihood of enzyme-substrate complex formation?
      More active sites are available for binding
    • What happens to the initial rate of reaction as enzyme concentration increases with sufficient substrate?
      The initial rate increases linearly with enzyme concentration
    • What occurs when substrate is limited and enzyme concentration is increased?
      Reaction rate does not increase due to substrate limitation
    • How does substrate concentration affect the rate of reaction?
      Higher substrate concentration increases reaction rate
    • What occurs when substrate concentration exceeds a certain point with fixed enzyme concentration?
      All active sites become saturated
    • What happens to enzyme-substrate complex formation as substrate molecules increase?
      Likelihood of complex formation increases
    • What happens when all active sites of enzymes are full?
      Additional substrate cannot bind to form complexes
    • What does the graph of reaction rate versus substrate concentration show?
      • Linear increase in reaction rate with substrate addition
      • Plateaus when all active sites are occupied
    • What happens when substrate concentration is continually increased with constant enzyme concentration?
      Enzyme active sites work continuously
    • What is Vmax in enzyme kinetics?
      Maximum possible rate of enzyme activity
    • What does it mean when substrate molecules are 'queuing up' for an active site?
      Enzyme is working at its maximum rate
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