Tissue Culture

• What is tissue culture

  Growth in an artificial medium of cells derived from living tissue
• What is plant tissue culture

  • Producing whole plants from tissue taken from parent plant
  • Plants produced will be clones
• Describe the steps of tissue culture

  • Choose parent plant with desired characteristics
  • Remove pieces of tissue from parent plant
  • (From roots or shoots of meristem)
  • Tissue added to growth medium containing nutrients + growth hormone
  • Tissue produces roots + shoots
  • Plantlet produced -> moved to potting compose to continue to grow
• What are the advantages of plant tissue culture

  • Only small amount of initial plant tissue needed
  • Grows in short amount of time
  • Clones -> has desired characteristics
  • Can be grown in a small space
  • Can grown all year around
• What is the issue with animal tissue culture

  • Can't be used to grow identical whole animals
  • Can be used to grow identical animals tissue
• Describe the steps for animal tissue culture

  • Get a sample of tissue from the animal with desired characteristic
  • Separate cells using enzymes
  • Place them in culture vessel containing growth medium
  • Grow cells + multiply through mitosis
  • Cells split up again + place in separate vessels for future growth
  • Once grown -> store for future use
• What are the advantages of animal tissue culture

  • Used in medical research -> can experiment however on tissues in isolation
  • Can look at effects + environmental changes without complications from other processes