Microscopy

• What should you be able to describe by the end of the video?

  Advantages of an electron microscope
• What is the main function of microscopes?

  To magnify images of specimens
• What did early microscopes look like?

  Similar to modern microscopes
• What important discoveries have light microscopes allowed us to make?

  Structures inside cells, like the nucleus
• What is a limitation of light microscopes regarding magnification?

  They have limited magnification power
• What is a limitation of light microscopes regarding resolution?

  They produce blurred images at high magnification
• Why was the electron microscope invented?

  To explore structures inside cells in detail
• What are the key advantages of electron microscopes?

  Greater magnification and resolution
• What can be seen in detail with an electron microscope?

  Details in the nucleus
• What is the formula for calculating magnification?

  Magnification = image size / real object size
• What will you be given in the exam regarding magnification calculations?

  The magnification equation
• If the image size of the nucleus is 45 mm and the real size is 0.01 mm, what is the magnification?

  4,500 times
• If the image size of a mitochondrion is 30 mm and the real size is 0.03 mm, what is the magnification?

  1,000 times
• How do you calculate the size of the real object if you know the image size and magnification?

  Real size = image size / magnification
• If the image size is 87 mm and the magnification is 2,000 times, what is the real size of the cell?

  0.435 mm
• What are the advantages of using an electron microscope over a light microscope?

  • Much greater magnification
  • Higher resolution
  • Ability to see fine details in specimens
• What steps should you follow to calculate magnification?

  1. Measure the image size
  2. Measure the real object size
  3. Use the formula: Magnification = image size / real object size
• How do light microscopes and electron microscopes differ in their capabilities?

  • Light microscopes: Limited magnification and resolution
  • Electron microscopes: High magnification and resolution