magnification core practical

• field of view

  the circular area you can see in a light microscope
• What should you wear when preparing onion cells for observation?

  Gloves
• Why is iodine solution used on the microscope slide?

  It stains the onion cells for better visibility
• What tool is used to add iodine solution onto the slide?

  Pipette
• What should be removed from the onion before placing it on the slide?

  A very small piece of skin
• What is the purpose of breathing on the slide before wiping it?

  To remove any fingerprints from the slide
• What should you do after placing the small piece of skin on the drop of iodine?

  Place a coverslip on the slide
• How should the coverslip be lowered onto the drop?

  Use a toothpick to avoid trapping air bubbles
• What should you do if liquid spreads out under the coverslip?

  Touch a piece of paper towel to it
• What is the first step in observing the slide under the microscope?

  Use the lowest magnification objective lens
• What should you do after using the lowest magnification to observe the slide?

  Switch to a higher magnification for detail
• What are the steps to prepare onion cells for observation under a microscope?

  1. Wear gloves.
  2. Use a pipette to add iodine solution.
  3. Remove a small piece of onion skin.
  4. Breathe on the slide and wipe it.
  5. Place the skin on the iodine drop.
  6. Lower a coverslip using a toothpick.
  7. Touch paper towel to any liquid.
  8. Start with the lowest magnification.
  9. Switch to higher magnification for detail.
• why should sample of animal cells be disposed of in disinfection?

  they could be contaminated with bacteria which could spread