plasmid removed from bacterium
insulin gene cut out human using restriction enzymes - restriction enzymes recognise specific sequences in DNA and cut DNA
one of DNA strands left with unparied bases - sticky bases
plasmid cut open using restriction enzyme leaving sticky ends
plasmid and human gene mixed
ligase added and joins sticky ends to produce recombinant DNA
recombinant DNA inserted into a bacterium
bacterium grown in vat to produce insulin