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Microscopes w1
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Cards (17)
what is magnification determined by
lens
ability to bend light
what happens when light ray passes through mediums
ray bends at interface, causing
refraction
what is the reflective index
measure of how great a
substance
slows
light
what is the objective lens and its role
collects light passing through
specimen
and focuses light beam for
magnified
image
why do we use oil for x100 oil immersion lens
refractive index
of
air
and glass are slightly different, so the oil bridges the gap
what is the equation for magnification
magnification= magnification
eyepiece
x objective lens
what is the nose piece also known as
revolving turret
the
stage
has a hold called the
aperture
for the illuminating beam to pass
what is the role of the coarse knob
bring
specimen
near to focus
what is the role of the fine knob
focus
quality
sharpened
what is the illuminator
the
light source
at the base of the microscope.
halogen bulbs
are often used
what is the condenser
lens
used to collect and focus light from the
illuminator
to the
specimen
, found under the stage
what is the iris diaphragm
below the
condenser
, above the
light source
. used to change
intensity
and
size
of light
what is the purpose of kohler illumination
provides
optimum
contrast
and
resolution
by focusing
object
, spreading
evenly
steps for Kohler illumination
place slide on stage and focus at 4x
focus specimen
close down condenser diaphragm
close illuminator so you should see 20p
focus with condenser knobs so 20p edges become sharp
move 20p to centre using screws
open diaphragm
open illuminator iris until full view
adjust condenser iris for better contrast
how to use the vernier scale
look where the 0 is
if it is between 10 and
10.1
, its 10
then when scale marries up, chose that. meaning if its at 3 it is
10.3
what technique can be used to view full slide
battlement
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