Testing For Proteins

• what test do you use to test for proteins?

  biuret test
• what does biuret test test for?

  proteins
• how many stages does the biuret stest have?

  2
• Give details about the BIURET TEST?

  1. The test solution needs to be ALKALINE, so first add a few drops of SODIUM HYDROXIDE SOLUTION
  2. Then add some copper (II) sulfate solution
• Why do you first add a few drops of sodium hydroxide solution to the test solution?


  because the test solution needs to be alkaline
• What happens if protein is present?


  the solution turns purple
• what happens if protein is NOT present?

  the solution will stay blue
• why do you need to look carefully at the results of the biuret test?

  because the colours are pale