Microscopy

• Which two lenses on a microscope are used to magnify the specimen?

  Eyepiece lens and Objective lens
• How do you calculate the magnification?

  magnification of eyepiece x magnification of objective
• How do you calculate the magnification of an image?


  size of image/real size of object
• Convert mm to um

  1mm - 1000 um

  100mm - 100,000 um
• Name the two types of electron microscopes.

  Scanning electron microscope and transmission electron microscope
• What are SEM’s used for?

  Examining the surface structure of specimens. Used at lower magnifications and have a large field of view.
• What are TEM’s used for?

  Examining thin slices or sections of cells or tissues
• Define magnification.


  A value that tells us how many times bigger an image is compared to the real object
• Define resolution 


  The ability to see detail and observe structures as separate objects
• State an advantage of using an electron microscope than a light microscope

  Has a higher resolution and a magnification, meaning that cells can be studied in finer detail. This enables biologists to see and understand more sub cellular structures
• What is the function of the coarse focus on a microscope?

  Can bring the stage closer to the objective lens to focus.
• What is the function of the fine focus on a microscope?


  Brings the slide back into focus.
• What are some risks when using a light microscope?

  Care must be taken when looking through the microscope if the illumination is too bright

  Care must be taken when using microscope stains

  Care must be taken when handling coverslips and microscope slides.