week1-equations

• What is the title of Lab Exercise #1?

  Familiarization with basic calculations
• What is the first material listed for the lab?

  Pipetman (P1000, P100, P10)
• Why are micropipettes commonly used in laboratories?

  They transfer precise, small liquid volumes
• What unit is used to measure the volume transferred by micropipettes?

  Microlitres (μL)
• What happens if you dial the pipette beyond its limit?

  It may break or become unreliable
• What are the two stops on the pipetting button used for?

  First stop draws liquid, second dispenses
• What are the three pipette models mentioned?

  P10, P100, P1000
• What is the maximum volume the P1000 pipette can carry?

  1000 μL
• How can you check the accuracy of a pipette?

  By weighing the dispensed water
• What is the density of water used for pipette accuracy checks?

  1 g/mL
• What is a solution in chemistry?

  • A homogenous mixture of substances
  • Forms a single phase
  • Cannot differentiate substances within it
• What is the term for the substance that is dissolved in a solution?

  Solute
• What is the term for the substance in which the solute is dissolved?

  Solvent
• What is an aqueous solution?

  A solution where water is the solvent
• What does dilution do to the concentration of a solute?

  It decreases the concentration of the solute
• What remains constant during dilution and concentration?

  The amount of solute stays the same
• What is the formula for calculating dilutions?

  C1 * V1 = C2 * V2
• What does C1 represent in the dilution formula?

  Concentration of stock solution
• What does V2 represent in the dilution formula?

  Final volume of new solution
• How do you prepare a 0.25 M solution from a 1 M solution?

  Use 1.25 mL of the 1 M solution
• What is the first step in preparing a dilution?

  Determine the volume of stock solution needed
• What are the steps to prepare a dilution?

  1. Calculate the volume of stock solution (V1)
  2. Measure the stock solution
  3. Add diluent to reach final volume (V2)
• What is the % by mass concentration formula?

  % By mass (w/w) = (mass of solute / total mass) * 100

  
• What is the % by volume concentration when 50 mL of pure alcohol is diluted to 100 mL?

  50% pure alcohol (v/v)
• What is the % weight by volume concentration formula?

  % weight by volume (% w/v) = (mass of solute / volume of solution) * 100

  
• What is the Molarity of a solution when 3 mol of CaCl2 is dissolved in 100 mL?

  30 M CaCl2

  
• What are the exercises related to concentration calculations?

  1. % by mass concentration of NaOH
  2. % by volume concentration of alcohol
  3. % weight by volume concentration of NaCl
  4. Molarity of CaCl2 solution

  
• What is the first step in calculating molarity?

  Convert mL to L

  
• What is the significance of knowing how to prepare aqueous solutions in biochemistry?

  Essential for studying biochemical processes