microbiology

• What type of bacterial culture is being used in the practical?

  A safe strain of E. coli
• Why must you handle the bacterial culture as if it were dangerous?

  To maintain safety and sterility
• What is the first step before handling the microbe?

  Wash your hands thoroughly
• Where should the agar plates be opened during the procedure?

  Near the Bunsen burner
• What should you do if you need to open the agar plate?

  Open it for the minimum time
• How should the agar plate be labeled?

  Label the bottom, not the lid
• Why is it important to write the date on the agar plate?

  To track how old the culture is
• What is the purpose of the Bunsen burner in the procedure?

  To create a sterile environment
• What is the first step in inoculating the agar plate?

  Use a sterile pipette
• How is the pipette sterilized before use?

  Wrapped in red paper and heated
• What should you do after taking bacteria from the bottle?

  Flame the lid before closing
• What is the purpose of spreading the bacteria on the agar plate?

  To ensure even growth
• What happens if you break the surface of the agar?

  Bacteria won't grow correctly
• What is used to kill any remaining bacteria on the spreader?

  Vinegar solution
• What is a lawn of bacteria?

  A dense growth of bacteria on agar
• What is the purpose of the antibiotic disk in the experiment?

  To test its effectiveness against bacteria
• How do you identify which disk is which on the agar plate?

  Label them as 1, 2, 3
• What should you do after placing the disks on the agar plate?

  Incubate the plate
• What indicates that the antibiotic is effective?

  A clear ring around the disk
• What is the significance of measuring the diameter of clear areas?

  To compare the effectiveness of treatments
• What factors should be considered for a fair test in this experiment?

  Viscosity of liquids and diffusion
• Why is it important to consider real-world applications of the results?

  To understand treatment options for infections
• What should be done after the experiment is completed?

  Disinfect the work area
• Why do we store agar plates upside down?

  To prevent condensation on the agar
• What happens to the agar plate after a few days?

  The bacteria grow and cloud the agar
• What does a cloudy area on the agar indicate?

  Growth of individual bacteria colonies
• What is the role of the teacher after the agar plate is prepared?

  To store it in an incubator