What is microscopy?

• What does microscopy refer to?

  Use of microscopes
• What are the main components of a light microscope?

  Base, arm, light source, stage, lenses
• What supports the entire light microscope?

  The base at the bottom
• What is the function of the light source in a microscope?

  To illuminate the object being viewed
• What is the stage of a microscope used for?

  To hold the microscope slide
• How many objective lenses are typically found in a light microscope?

  Three objective lenses
• What are the typical magnifications of objective lenses?

  10x, 20x, and 50x
• What is the purpose of the eyepiece lens in a microscope?

  To look into the microscope
• What are the coarse and fine focusing knobs used for?

  To help focus the image
• What does the term 'object' refer to in microscopy?

  The real object being observed
• What does the term 'image' refer to in microscopy?

  The visual representation seen through the microscope
• How does light travel through a light microscope?

  It reflects through the object and lenses
• What is magnification in microscopy?

  How many times larger the image is than the object
• What is the equation for magnification?

  Magnification = image size / object size
• What is resolution in microscopy?

  The shortest distance between distinguishable points
• How does resolution affect the clarity of an image?

  Higher resolution means more detail and less blur
• How do two images of the same magnification differ in resolution?

  One may appear blurrier than the other
• What happens to the level of detail as resolution increases?

  More details become visible
• What are the key components of a light microscope and their functions?

  • Base: Supports the microscope
  • Arm: Connects top and bottom
  • Light source: Illuminates the object
  • Stage: Holds the microscope slide
  • Objective lenses: Magnify the object
  • Eyepiece lens: Where we look into
  • Coarse and fine knobs: Focus the image
• What are the definitions of object, image, magnification, and resolution in microscopy?

  • Object: The real sample being observed
  • Image: The visual representation seen
  • Magnification: Size of image compared to object
  • Resolution: Shortest distance between distinguishable points