tissue culture

• what are the methods of plant cloning

  
  

  Taking plant cuttings

  Tissue culture
• Tissue culture


  A method of growing living tissue in a suitable medium
• Growing plants using tissue culture

  1. Select a plant that shows desired characteristics

  2. Cut multiple small sample pieces from meristem tissue

  3. Grow in a petri dish containing growth medium

  4. Transfer to compost for further growth
• Ensure aseptic conditions to prevent contamination by microorganisms when preparing tissue cultures
• Growth medium

  Contains nutrients and growth hormones
• Advantages of growing plants by tissue culture

  • simple and fast process , can grow plants quickly
  • Enables the growth of many plant clones with the same desirable characteristics
  • Useful in the preservation of endangered plant species
• Disadvantages of growing plants by tissue culture

  • Reduction in the gene pool
  • Plant clones often have a low survival rate
  • Could unknowingly increase the presence of harmful recessive alleles
• Preparing animal tissue cultures

  1. Extract a sample of tissue from an animal

  2. Use enzymes to separate cells within the sample

  3. Grow in a culture vessel containing growth medium

  4. Once grown, store the sample