w15 ELISA

    Cards (25)

    • what does ELISA stand for
      enzyme linked immunosorbent assay
    • what does FeLV stand for
      feline leukaemia virus
    • is FeLV seen on wild or domestic cats 

      both
    • what type of virus is FeLV
      retrovirus
    • what type of transmission is FeLV
      Oranasal e.g. grooming, shared bowls
    • what are the signs of FeLV
      immunosuppressant
      tumours
      anaemia
      reproductive problems
      enteritis
      urinary incontinence
      uveitis
    • what is a retrovirus
      virus that invades cells and attacks the DNA causing it to change. these cells are then more likely to replicate and form cancer
    • what is uveitis
      inflammation of structures of the eye
    • what are the ways to diagnose FeLV
      ELISA test
      immunofluorescence assay
      virus isolation
      PCR
    • what are the advantages of using ELISA
      quick
      specific
    • what is the meaning of specific in terms of testing
      low number of false positives
    • how does the immunofluorescence assay work
      antibody attaches to fluorescent substance, use UV light to see
    • what is the disadvantage to immunofluorescence assay
      it is expensive
      needs to be send to lab so delayed test
    • when do we use immunofluorescence assay
      if we get a positive ELISA test
    • when should the ELISA test be carried out
      when a cat is entering a high population e.g. rescue centre
      before vaccine against FeLV
      cats being imported to the UK
    • what may happen if you test for FeLV after giving the vaccine
      may give a false positive
    • what does the ELISA test detect
      presence of antibodies or antigen
    • what are the steps for ELISA testing
      1. Add sample of antigen so they stick to wall
      2. add primary antibodies should be complementary in shape
      3. wash
      4. add secondary antibody with enzyme attached to bind to primary antibody
      5. add substrate for secondary antibody to work on showing colour change
    • what is an indirect ELISA test
      needs 2 antibodies for test
    • what is a direct ELISA test
      all done in one step as primary antibody has enzyme on it
    • what does a quantative ELISA test tell us
      yes or no if antigen is present
    • where are quantative ELISA tests used
      in house
    • what do qualitative ELISA tests measure
      degree of colour change indicating how many antigens present
    • where are qualitative ELISA tests performed
      in lab
    • what could happen in you contaminate ELISA test
      give false positive
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