Save
ADT
w15 ELISA
Save
Share
Learn
Content
Leaderboard
Share
Learn
Created by
Lily
Visit profile
Cards (25)
what does ELISA stand for
enzyme linked immunosorbent assay
what does FeLV stand for
feline leukaemia virus
is
FeLV
seen on
wild
or
domestic
cats
both
what type of virus is FeLV
retrovirus
what type of transmission is FeLV
Oranasal
e.g.
grooming
,
shared
bowls
what are the signs of FeLV
immunosuppressant
tumours
anaemia
reproductive problems
enteritis
urinary incontinence
uveitis
what is a retrovirus
virus that
invades
cells and attacks the DNA causing it to change. these cells are then more likely to
replicate
and form
cancer
what is
uveitis
inflammation
of structures of the eye
what are the ways to diagnose FeLV
ELISA
test
immunofluorescence assay
virus isolation
PCR
what are the advantages of using ELISA
quick
specific
what is the meaning of specific in terms of testing
low number of
false positives
how does the immunofluorescence assay work
antibody
attaches to
fluorescent substance
, use
UV light
to see
what is the disadvantage to immunofluorescence assay
it is
expensive
needs to be send to
lab
so
delayed test
when do we use immunofluorescence assay
if we get a positive
ELISA
test
when should the ELISA test be carried out
when a cat is entering a high population e.g. rescue centre
before vaccine against
FeLV
cats being imported to the
UK
what may happen if you test for FeLV after giving the vaccine
may give a
false positive
what does the ELISA test detect
presence of
antibodies
or
antigen
what are the steps for ELISA testing
Add sample of
antigen
so they stick to wall
add primary
antibodies
should be complementary in shape
wash
add secondary
antibody
with
enzyme
attached to bind to primary antibody
add substrate for secondary antibody to work on showing colour change
what is an indirect ELISA test
needs 2
antibodies
for test
what is a direct ELISA test
all done in one step as
primary antibody
has
enzyme
on it
what does a quantative ELISA test tell us
yes or no if
antigen
is present
where are quantative ELISA tests used
in
house
what do qualitative ELISA tests measure
degree
of colour change indicating how many
antigens
present
where are qualitative ELISA tests performed
in
lab
what could happen in you contaminate ELISA test
give
false positive
See similar decks
6.1.10 "Elise ou la vraie vie" by Claire Etcherelli
AQA A-Level French > 6. Works: Literary texts and films > 6.1 Literary texts
104 cards
ELISA
35 cards
Elisa
12 cards
elisa
1 card
ELISA
Help files
31 cards
ELISA
My Biology A-level > Cells > Immunity and cell recognition
4 cards
ELISA
Biology > Unit 2: cells > 08 cell recognition and immune system > 04 antibodies and monoclonal antibodies
12 cards
ELISA
BMS3008 Integrated Biomedical Sciences
4 cards
ELISA
Biology
17 cards
ELISA
TB2 > formulation and analytical chemistry
22 cards
ELISA
Biology > CELLS > MONOCLONAL ANTIBODIES & ELIZA
3 cards
ELISA Test
7 cards
ELISA Principles
9 cards
ELISA
Biomolecules (BB4BMC) > Year 1 Content > Biochemical Methods
5 cards
ELISA
ADT
24 cards
ELISA
CMB2000
30 cards
ELISA test
24 cards
ELISA Test
13 cards
ELISA Tests
Biology
5 cards
Biology Elisa
22 cards
Elisa test
8 cards