RP01 microscopy

• which microscope is used for this practical?

  light microscope
• What is the slide?


  A small sheet of glass or plastic
• light microscope diagram

  A) Eyepiece

  B) High and low power objective lens

  C) Stage

  D) Light

  E) Fine adjustment knob

  F) Coarse adjustment knob

  
• Method for using a slide with onions

  Add a drop of water to the middle of a clean slide

  cut up an union into thin layers

  Use tweezers to peel off some epidermal tissue

  Place the epidermal tissue on the slide

  Add a drop of iodine solution

  Place a cover slip on top and put under lens
• why is iodine used?

  it is used as a stain

  highlights an object in a cell by adding colour
• Which objective lens has the lowest magnification?

  shortest objective lens
• what word is used to describe placing the object on the slide?

  mounting
• what magnification is usual for the eyepiece lens?

  x10 magnification
• why may you look at the slide from the side?

  to stop the lens from hitting the slide
• how can the illumination be altered?

  the dial on the side of the microscope
• method for using the microscope to look at slide

  clip the slide

  use the lowest powered objective lens

  look at eyepiece use the coarse adjustment know to move the stage up and down until image is focused

  adjust the focus with the fine adjustment knob until a clear image

  you can use a higher powered objective lens for higher magnification